The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. Get pricing and purchase lab equipment and. 4. Model : VersaMax . Full size table. 635. The absorbance was read at 570 nm using a spectrophotometer (VersaMax, Molecular Devices, San Jose, CA, USA). Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. 00 USD. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. A standard curve, provided by the manufacturer, was generated and the sample concentrations determined by 4-parameter logistic curve. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. GABA concentrations in samples were calculated from calibration curves using the standard solutions. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 9000-0756. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Molecular Devices Spectramax Plate Reader. Monday to Friday 7am - 5pm Pacific Time. Molecular Devices VersaMax Tunable Microplate Reader. Software (optional), such as Softmax Pro (Molecular Devices). A series of 44 excipients were evaluated at six different concentrations in triplicate. Nude mice were obtained from Orient Bio Inc. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The. , VersaMax; Molecular Devices) Incubator for cell culture. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. The. The emodin thermoreversible PG was. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. VersaMax ELISA Microplate Reader. HT22 cells are derived from hippocampal neuronal HT4 cells. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 0. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). Cell viability was expressed as percent survival of untreated cells. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. Molecular Devices, LLC. The absorbance was measured at 570 nm using a microplate reader (VersaMax, Molecular Devices). To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. For wells that were not exposed to dehydration (positive control), all the cells (100%) survived for the 60-min test. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Taking into account the price differences between each seller and each country. Whether bacteria were partially or completely inactivated by bone cement. 72 MB. Antioxidant enzyme activityProtein concentration was determined from diluted crude venom and nanoparticle samples (1 mg/mL) in deionized water by BCA™ Protein Assay using a UV/V spectrophotometer (VersaMax, Molecular Devices, CA, USA) at a wavelength of 562 nm. Ann Arbor, MI, USA). The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. E. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. , Sunnyvale, CA, USA). SpectraMax® i3 Multi-Mode Detection Platform. 0 out of 5 $ 2,999. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Data were normalized to those of the PBS exposure control group. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. Culture medium was removed, and lysis buffer (10 ml) was pipetted into each well. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. HepG2 cells were seeded into 6-well plates. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. 3 Methods 1. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. The growth inhibitory rate (GIR) of treated cells was calculated by Equation 1. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 5% NP-40) supplemented with protease and phosphatase inhibitor cocktail (GenDepot, Baker, TX, USA). Model: VERSAmax Tunable Microplate Reader. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Open the Windows Task Manager, go to the Services tab, and click Open Services. Cell viability was assessed using the WST-1 assay. . Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. 1 software, the leading microplate data acquisition and analysis software, you can download this PDF file from Molecular Devices. , Spectramax Gemini; Molecular Devices) Tissue culture plates (24- and 96-well) Vacuum suction pumpRelated products. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. If you are looking for a comprehensive user guide for SoftMax Pro 7. Data were analyzed with GraphPad Prism. Cellular imaging was performed on a Confocal microscope (Olympus IX 81 + FV1000, Shanghai, China) using an excitation wavelength of 380 nm. Cell viability was analyzed using a cell counting kit assay (Dojindo). Accession codes. Unit price / per . Glossary of Terms. Molecular Devices VersaMax Microplate Reader. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Molecular Devices SpectraMax Plus 384 Microplate Reader *Parts Only* Tecan Sunrise Microplate Reader. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. All the experiments were conducted at. We would like to show you a description here but the site won’t allow us. To purchase a newer software version, request a quote. Sale Sold out. We would like to show you a description here but the site won’t allow us. 파장 범위(nm) : 340–850. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). We would like to show you a description here but the site won’t allow us. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). 9. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. Sale. 47 shipping. The narrowly focused light beam and optical design give you the same high performance with round bottom, flat bottom, or half area well plates. 4. 0 nm full width half maximum. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. At 48 h after transfection the cells were exposed to CoCl 2 (200 µM) or vehicle. That's why our team is at your disposal the majority of time. 3 Methods 1. Click SoftMax Pro Software User Guide. 0 out of 5 $ 2,999-20%. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. To update the driver. 23,225, Thermo Fisher Scientific). All analyses were performed three times, and the mean values were used for further calculations. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. 8). This. Thus, the molecular mechanism promoting hepatic lipid accumulation in obesity is of biomedical and public health interest. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Results of host range test of Mae-Yong924-1 against. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. BMG Labtech - THERMOstar. S. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. SpectraTest ABS1 Absorbance Validation Plate. MDC instruments including: SpectraMax®, VersaMax. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). VERSAMAX. The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. . Get pricing and purchase lab equipment and. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. To update the driver. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). 1 mL. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. Windows 10 – 2004/20H2/21H1/21H2. Select Start>Control panel>Device Manager. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. A. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). $495. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. 10. BMG Labtech - THERMOstar. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. Molecular Devices SoftMax Pro 5. S. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. ¨We would like to show you a description here but the site won’t allow us. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. Monochromator, tunable in 1-nm increments. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Enzyme activity was. . Offer Ends In: 5 Days 05: 02: 03. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). The absorbance was measured at 570 nm and. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 3, Molecular Devices). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Bimolecular fluorescence complementation (BiFC) systemMolecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. 8. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . Tested w/warranty. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Supplier: Molecular Devices. Reboot the computer. This document is provided to customers who have purchased Molecular Devices, Inc. Never order or replace filters to optimize an assay again! Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. S. OptiMax (Versamax non EXT) B0: Screenstation: all: Skanstacker: all: Skanwasher 300: all: Skanwasher 400: all: SpectraMax 190: N0:. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Absorbance plate reader (e. This reliable model allows for. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). 0 OD. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Versamax Microplate Reader dibangun di sekitar monokromator kisi. Select Update Driver. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. 파장 정확도 : < ±0. $1,749. spectrophotometer (VERSAmax, Molecular Devices). Fluorescence-activated cell-sorting analysis For analysis of DC surface molecules, 5×10 5 DCs were treated with soluble OVA (20 μg/mL), PLGA NPs (20 μg/mL OVA), PEI-coated PLGA NPs (20 μg/mL OVA), or 1 μg/mL. Narrow bandwidths deliver increased accuracy and enhanced peak. reader (VersaMax, Molecular devices, San Jose, CA, USA). Unit price / per . The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Turn the reader on and secure the USB cable. A. Tunable, filterless flexibility The. This manual does not represent a commitment on the part of Molecular Devices Corporation. from Molecular Devices Corporation. After treatment with GA3The absorbance was measured with a microplate reader (VERSAmax, Molecular devices, San Jose, CA, USA) at 570 nm according to the manufacturer’s instructions. All animal experiments were performed in compliance with the relevant laws and. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cell cycle investigation. After freeze-drying, the. 1 M sodium phosphate buffer (pH 6. The IC 50 value was generated from the log dose-response curves for cells using the Graphpad Prism version 9 for Windows (Graphpad Software, La Jolla, CA, USA). SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The cellular viability. For more information, please visit us at With the VersaMax microplate reader, you will never have to buy another filter. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). No accessories are needed and SoftMax® Pro Software. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. 99 shipping. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). Molecular Devices also offers a mass spectrometry-based solution for food safety testingWe would like to show you a description here but the site won’t allow us. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). 4 (Molecular Devices cat. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. MiR-21. Using a Spectradrop Micro-Volume Microplate (VersaMax, Molecular Devices, CA, USA), RNA was quantified, and 1 μg of total RNA was treated with DNase I (Thermo Scientific, Waltham, MA, USA) before. MDC instruments including: SpectraMax®, VersaMax. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. Then, the cultured cells were treated with different. 68 $. Finally, 0. 0 (Molecular Devices, Sunnyvale, CA). SpectraTest ABS1 Absorbance Validation Plate. ) for the next 36 h and provided a time-proliferation curve as a test result for each well of the microplate. Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. Software (optional), such as Softmax Pro (Molecular Devices). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. , Sunnyvale, CA, USA). Lihat Video. Select up to two wavelengths. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. The assays were conducted in a 96-well microplate using an ELISA microplate reader (VersaMax Molecular Devices, United States) to measure absorbance at 475 nm. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 747. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. 635. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). 00 USD. . The index was evaluated at 48 and 72 hours normalized to that at 24 hours. The limit of detection for each assay was 156. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. 6/10 #0120-1330d. After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. 00 USD. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. 0A Sunnyvale, CA Made in USA The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. S. 1. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). 17. An ELISA plate reader (VERSAmax; Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at 480 nm. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. Table 1. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. StakMax. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. Cells were lysed in NETN buffer (100 mM NaCl, 20 mM Tris pH 8. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. P. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. New and used Molecular Devices VersaMax's for sale on LabX. $199. FRAP solutions were prepared as described previously (Benzie and Strain, 1996, Benzie and Strain, 1999). Conventions Used in this Manual. Values are presented as the mean ?standard deviation (SD). Ann Arbor, MI, USA). 3. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Next, 100 μL of a reagent was added to an equal volume of cell culture medium present in each well. The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 9000-0756. Measurement of changes in BDNF production and release was done byMicroplate Readers. +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. We would like to show you a description here but the site won’t allow us. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. Wavelength Selection. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). 00. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Access all the resources, tools and information you need to stay productive. Table of Contents. The sections were stained with hematoxylin and eosin (H&E) and. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. (JC-1) (Molecular Probes), respectively. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. (Seongnam, Korea). Select Start>Control panel>Device Manager. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. ). SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. The final mixtures were allowed to react at 25 °C in the dark for 5 min. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. Measurement of biofilm formation inhibitory activity. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. Taking into account the price differences between each seller and each country. Cell proliferation assay MDA-MB-231 cells were seeded at a density. 0 N sulfuric acid. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). To make your request, nothing more simple: you just have to connect on the site and to make your request on line. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. This technical note is designed to provide information . 003 OD ±1. #VERSAMAX) SoftMax Pro GxP Software version 5. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website.